Bacterial stalk rot of maize caused by Dickeya zeae previously known as E. chrysanthemi pv. zeae have economic importance of reduced crop yield up to 98.8%. The disease is more prevalent in rainy season in India. The bacterium prefers high temperature and moisture for their growth result is plant toppled down within week. Plant growth promoting rhizobacteria (PGPR) are able to stimulate growth and resistance against plant diseases when they are able to have a positive effect on the plant health, and then demonstrate good competitive qualities and capabilities over existing rhizosphere communities. Ten rhizobacterial isolates were isolated uing nutrient agar and TSA and Kings B media from maize rhizospheric soil at Indian Agricultural Research Institute Delhi and KVK, Dhaulakuan Himachal Pradesh. These rhizobacterial were molecular characterized and identified by using 16S rRNA sequencing, namely B. cereus (JCM2152), Bacillus stercoris (D7XPN1), B. stercoris(JCM 30051), B. vallismortis. (NBRC101236), B.rugosus (SPB7), B. rugosus (SPBP), B. velezensis(CBMB205), B. wiedmanni (FSLW8-0169), Calidifontibacillus erzurumensi (P216S), Priestia endophytica(2DT). Phylogenetic tree grouped these isolates into two groups. The 16S rRNA sequence of strain JCM2152 was found 99.36% higher identical to Bacillus cereus. Strain NBRC101236 was found 92.70% lower similarities to Bacillus vallismortis.