The present study aimed to isolate and characterize the black rot pathogen, Xanthomonas campestris pv. campestris (Xcc), from cabbage, broccoli, radish, mustard, cauliflower and Knolkhol in the EKH district of Meghalaya, India. A total of 20 isolates were obtained, and 16 of these were confirmed to be pathogenic based on the development of characteristic "V-shaped" lesions. From these 16 isolates, 6 were selected from each crucifers’ strains for further biochemical and molecular studies. Microscopic and cultural studies revealed that all 6 isolates were gram-negative, rod-shaped bacteria with circular colonies. However, variations in texture, margin, consistency, color, and growth pattern were observed among the isolates. For instance, isolate XC CAB4 exhibited a smooth texture, lobate margin, slimy consistency, and pale-yellow color, while isolate XC CALI1 had a less smooth texture, an entire margin, a more slimy and less glistening consistency, and a deep yellow color. Biochemical tests showed that all 6 isolates were positive for H2S production, starch hydrolysis, gelatin liquefaction, and ammonia production, but negative for indole production, casein hydrolysis, Voge's Proskauer test, and methyl red test. The isolates were further confirmed as Xcc by amplifying the hrp F gene using specific primers, and a phylogenetic tree was constructed to compare the isolates with other representative strains. Cross-pathogenicity and aggressiveness assays revealed that all 6 isolates were capable of infecting different cruciferous crops, with varying infection rates and progression of black rot disease. The area under the disease progress curve (AUDPC) was calculated for each isolate, with XC BRO1, XC CALI1, and XC MSD2 showing higher AUDPC values, indicating greater aggressiveness in causing black rot disease on cabbage leaves compared to XC CAB4, XC RAD2, and XC KNKH1.These findings enhance our comprehension of the virulence of various Xcc isolates from cruciferous crops in Meghalaya. This understanding can aid in screening and developing cruciferous cultivars that are resistant to Xcc, predicting disease outbreaks, and evaluating the risk of crop damage. It also aids in the development of effective strategies for managing black rot disease in cruciferous crops.