Mitigation of water stress by the application of brassinosteroids (BRs) on seedlings of Eucalyptus tereticornis was undertaken in the screen house at CCS Haryana Agricultural University, Hisar. BRs are polyhydroxylated steroidal phyto-hormones, which play an important role in regulation of various plant growth and developmental processes. Keeping in view the future prospects of Eucalyptus tereticornis in agroforestry areas, the present study was carried out on the application of brassinosteroids for raising healthy seedlings for the purpose of plantations on private and public lands. At moisture level of field capacity, growth of plants was found maximum and it decreased as the water stress increased. Foliar application of 5 ppm of BRs at 75% moisture of field capacity, 10 ppm at 60% of field capacity and 20 ppm at 60% and 40% moisture levels were found most suitable to mitigate the water stress in Eucalyptus tereticornis.