The study reports the isolation and identification of the dermatophyte Microsporum canis by mycological culture of skin scabs and hair plucks collected from dogs and cats with dermatological complaints and presented to the Centralized Clinical Laboratory, Madras Veterinary College, Chennai-07. M. canis was successfully isolated from 64 canine and 16 feline patients which accounted to population incidence of 4.31% in canine and 72.72% in feline population. Breed-specific analyses in dogs indicated the highest prevalence in Labrador and non-descript breeds, while sex-based analysis showed a predominance in males, and age-wise distribution revealed a peak in the 1-5 year age group. Similarly, in cats, native Indian breeds, females, and individuals aged 1-5 years exhibited the highest incidence. A notable seasonal trend was observed, with the highest occurrence of M. canis recorded during the summer season in both dogs and cats.