The present study aimed to isolate, characterize, and evaluate native Trichoderma isolates for the eco-friendly management of wilt disease of chilli caused by Fusarium solani, which is considered the most common fungal species associated with chilli. Chilli wilt disease is a significant threat worldwide, resulting in 50-80% yield losses during severe outbreaks. In the present investigation, seventy soil samples were collected from seven districts of Uttarakhand, viz. Tehri, Nainital, Pauri, Bageshwar, Uttarkashi, Almora and Chamoli, and the elevation ranged from 1370 m to 3200 m above the mean sea level. The soil samples were used to isolate Trichoderma isolates, and the method adopted for isolation was the serial soil dilution plate method on the Trichoderma-specific medium (TSM). The Trichoderma isolates were characterized based on cultural, morphological and microscopic observations. Further, potential Trichoderma isolates were evaluated for their antagonistic activity, antibiosis activity, change in pH and phosphate solubilizing activity under in-vitro conditions. After in-vitro screening, seven putative novel Trichoderma isolates viz., TB1, TS26, TS18, TS23, TCH6, TDG, and TS40 were selected. Further, they were evaluated as bio-control agents using seed bio-priming technique for the wilt disease suppression in chilli caused by Fusarium solani under glasshouse conditions. In the present investigation, the performance of potential Trichoderma isolates was found to be significantly superior to T2 (Fusarium inoculated soil; Negative control) with respect to wilt disease suppression. Among all the treatments, T8 ( Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TDG) having a value of 90%, recorded a maximum Percent Disease Reduction (PDR), followed by T3 (Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TB1), with a PDR of 86.67% which was found to be statistically at par with T8 , while T9 (Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TS40), T4 (Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TS26), T6 (Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TS23) and T7 (Fusarium inoculated soil + Seed biopriming with Trichoderma isolate TCH6) were recorded with values of 73.33%, 70%, 70%, 66.67% and 63.33% PDR respectively. However, no PDR was recorded in T2 (Negative control). This study highlights the potential of Trichoderma spp. as a potential bio-control agent with a broad action spectrum and offers a sustainable alternative to fungicides for managing wilt disease in chilli in the hills of Uttarakhand.