Nutrient concentrations in plant tissues are often regarded as the most reliable measure of the nutritional quality of different crops. Leaf nutrition analysis is an excellent way to establish balanced fertilisation techniques. The current study seeks to assess macronutrient content in ten varieties of pot gerbera cultivated on three different growing conditions after 90 days of planting. The experiment was conducted in the polyhouse at the Centre for Protected Cultivation Technology (CPCT), ICAR-Indian Agricultural Research Institute (IARI), New Delhi, from February to May 2022 and 2023, using a completely randomised design with a factorial concept. The results showed that the leaves of var. Glorious Orange grown in Cocopeat + Perlite + Vermiculite (3:1:1) (M2V2) had the highest total leaf nitrogen content (2.45 %), whereas the leaves of var. Bighorn grown in Cocopeat + Perlite + Vermiculite (3:1:1) (M2V9) had the highest leaf phosphorous content (0.42 %). However, the leaves of var. Glorious Yellow exhibited the highest leaf potassium content (3.53 %) when grown in Cocopeat + Perlite + Vermiculite (3:1:1) (M2V4) than in any other treatment combination. There was no significant relationship between varieties and growing media.