Strawberry (Fragaria x ananassa Duch.) is an important table fruit of millions of people around the world for its nutrient content and pleasant flavour. The fruits are rich in ascorbic acid, secondary metabolites and simple sugars. It is grown extensively under temperate, sub-tropical and tropical climate throughout the year. During the cultivation practices of strawberry, the leaf spots are the major emerging diseases and the deleterious fungal pathogens found to attack the strawberry crop which affects the production and productivity. An extensive survey was conducted in three major strawberry growing districts of Meghalaya (India) during 2021-22. Leaf spot diseases were observed with a disease incidence of 16.29 to 46.66 % of Pestalotiopsis leaf spot and 11.11- 36.10 % of Alternaria leaf spot. A total of 22 isolates of Pestalotiopsis and 10 isolates of Alternaria obtained from different surveyed locations were isolated on the PDA medium. The microscopic observations of Pestalotiopsis showed fusiform conidia comprised of 5 cells with a dimension of 17.35-31.99 µm length and 4.88- 11.28 µm breadth. In similar way, Alternaria showed muriform conidia with a dimension of 13.75-25.13 µm length and 6.96-13.75 µm breadth with vertical and horizontal septations. Based on the cultural, morphological characters and molecular characterization, the pathogens were identified as Neopestalotiopsis clavispora and Alternaria alternata. The DNA sequences were submitted to NCBI portal and got the accession numbers for both the pathogens (OR539470 and OR540826). Pathogenicity tests conducted by inoculation with a conidial suspension of pathogens, produced typical leaf spot symptoms and the re-isolation of pathogens confirmed the Koch’s postulates. The documentation study of disease incidence and severity among different surveyed fields showed an importance of the pathogens and the yield losses caused by these pathogens. Hence an immediate research work has to be undertaken for the development of effective disease management strategies for improving the production and productivity of strawberry.