Lentil (Lens culinaris Medik), is widely grown pulse crop. Lentil crop might be attacked by several seed and soil borne diseases. In vitro bio-efficacy of different antagonists and organic amendments on mycelial growth of Fusarium oxysporum f. sp. lentis by using seven Trichoderma spp. (T. harzianum, T. viride, T. hamatum, T. asperellum, T. virens, T. koningii, T. atroviride) and seven OAs (vermicompost, FYM, mustard cake, goat cake, mungbean chickpea, cluster bean). In vivo estimation of microflora propagules density, growth, phenological, physiological (chlorophyll and carotenoid contents) biomass parameters and qualitative, yield contributing characters. Under in vitro, the highest mycelial growth inhibition (68.52%) was found with T. harzianum, and among OAs maximum mycelium inhibition was recorded with vermicompost (63.34%). In vivo study, T harzianum + vermicompost effectively reduces microflora propagules (23, 42, 45, 40×105cfu/g soil) at the different intervals (planting, 1, 2, 3, 3.5 months) than the other treatments. This combination enhances percent germination (96.67), phenological and biomass parameters like plant height (47.23 cm), pods/plant (60.42), seeds/plant and yield of seed/plant (4.45 g), fresh and dry weight of shoot & root (33.98, 2.89 and 6.97, 0.79 g) and reduces the disease incidence (14.88 %). Physiological pigments viz; chlorophyll (3.75 mg/ml) and carotenoids (0.71 mg/ml) also recorded maximum in T harzianum + vermicompost combination. The qualitative parameters like plant height (44.67 cm), root nodule (95.00), and yield contributing characters like pods/plant (59.00), seeds/plant (100.00), yield (430.00 kg/h) found maximum in this combination.