The present study was carried out to evaluate the antifungal efficacy of nine crude plant extract under in vitro conditions against fungal pathogen Colletotrichum gloeosporioides, the cause of anthracnose disease of mango and subsequently potential of efficacy of these bio-resources were also tested in two different geographical conditions of Himachal Pradesh. Aqueous extracts of different plant parts (leaves, fruits, and seeds) were used at concentrations of 10%, 25% and 50% (w/v), which amended with potato dextrose agar culture medium on which discs of C. gloeosporioides were inoculated using Poison food technique followed by incubation at room temperature for seven days. Therefore, the result of finding demonstrated that among all nine aqueous plant extracts, Azadirachta indica (Neem) exhibited 74.2 per cent as significantly highest mycelial growth inhibition which was followed by aqueous fruit extract of Emblica officinalis (Aonla) with mycelial growth inhibition of 62.4 per cent, respectively. However, aqueous leaf extracts of Murraya koenigii (Karipatta) was reported to be significantly least effective (40.4%) in inhibiting the mycelial growth of Colletotrichum gloeosporioides. This suggests that aqueous leaf extracts of neem attributed fungicidal properties against target pathogen, C. gloeosporioides. Additionally, potential antifungal efficacy of in vitro tested four most effective botanical comprised of Emblica officinalis, Azadirachta indica, Aloe barbendens and Melia azedarach were evaluated in two different geographical conditions of Himachal Pradesh viz. DhaulaKuan and Jachh. Under field conditions, among all treatments foliar sprays aqueous leaf extracts of A. indica (15%) exhibited significantly highest per cent disease control both on leaves (53.2%) and fruits (50.2%), respectively. From the present study, we can conclude that the aqueous leaf extract neem can be explored as effective eco-friendly alternative over hazardous synthetic fungicides to mitigate anthracnose disease incidence even at field conditions.