This investigation was carried out in well adopted buffalo breed Murrah over a period of nine months, encompassing three seasons, to demonstrate the effect of cumin and molasses supplementation on thermographic profile of Murrah heifers at different anatomical locations viz., forehead temperature (°C), ear temperature (°C), elbow joint temperature (°C) and flank temperature (°C) during different season. A total of 24 Murrah heifers, maintained at Kathura village of Sonipat district (India) were selected and monitored for the thermal profiling at different anatomical locations of body using infrared thermography at monthly interval during hot dry and hot humid season using an IR camera (ThermaCamTM SC2000;FLIR Systems, Inc., Wilsonville, OR, USA). Thermograms were analysed by ThermaCamTM Researcher 2001 software (FLIR Systems AB, Danderyd, Sweden). Statistical analysis revealed that different supplement and seasons had significant (P<0.05) effect on body surface temperature at different anatomical sites. The magnitude of the increase in the IRT temperature at different anatomical regions of Murrah heifers was lower in the treatment groups compared to control group. The results of the study suggest that cumin and molasses can be used to alleviate heat stress in Murrah heifers.