A field experimentation was undertaken during kharif 2019 at Instructional Farm of Agronomy, Rajasthan College of Agriculture, Udaipur to study the influence of weed management and phosphorus fertilization on nutrient content, uptake and biochemical parameters of cowpea (Vigna unguiculata (L.) Walp.). Outcome of this research revealed that maximum chlorophyll content, seed protein content, nitrogen and phosphorus content of seed and haulm and their uptake by seed, haulm and total uptake by cowpea crop were recorded with pre-emergence application of pendimethalin 750 g ha-1 in combination with imazethapyr + imazamox 33.75 g ha-1 as post-emergence at 15-20 DAS next to weed free treatment. Whereas, application of phosphorus at various doses influenced chlorophyll content, seed protein content, nitrogen and phosphorus content of seed and haulm and their uptake by seed, haulm and total uptake by cowpea crop significantly.