Sugarcane is an important annual crop mailed propagated by setts. But bud chips are used under Sustainable Sugarcane Initiative (SSI) which needs to vigourous seedlings. Therefore, the present experiment was conducted during the special season of 2020-2021 at Sugarcane Research Station, Cuddalore, Tamil Nadu this experiment was conducted to study the influence of combining different mediums like Cocopith, Vermicompost, Pressmud, Farm Yard Manure (FYM) on seedling vigour of sugarcane under Sustainable Sugarcane Initiative (SSI). The experiment was laid out in CRD replicated thrice with 8 treatments using CoC 13339 as test variety. The treatments consisted of combinations different combinations of cocopeat, vermicompost, compost pressmud and farmyard manure along with growth regulators. The germination percentage, root shoot ratio, seedling vigour and chlorophyll content were observed. It was concluded that pre-plant treatment of sugarcane bud chips with plant growth promoters NAA 1mM and CaCl2 1mM and 75% coirpith along with 25% vermicompost improved sprouting percentage and seedling establishment. Hence, it improved the performance of novel bud chip technology for producing vigorous seedlings.