To better understand the impact of salicylic acid and sodium nitropruside on banana cultivars we were conducted an experiment. In this experiment salicylic acid 750 µM and 1000 µM, sodium nitropruside 0.5 mM and 1 mM were sprayed on Grand Naine and Tella Chakkerakeli banana bunches at 15 days prior to harvest. After harvest banana hands were kept for storage studies at ambient room temperature (25±2°C and 70±2 % RH). The results indicated that Grand Naine banana (shelf life 15.1d) fruits had responded to ethylene inhibitors considerably as compared to Tella Chakkerakeli banana (shelf life 8.5d). However, among different pre harvest treatments, salicylic acid @ 1000 μM followed by salicylic acid @ 750 μM were found to be more effective in retention of cell wall degrading enzyme such as pectin methylestarase and antioxidant enzymes, peroxidase and catalase. Ethylene biosynthesis (MaACO1, MaACO2, MaACS1, MaACS2, MaACS3 and MaACS4), ethylene receptor (MaERS1) and ethylene transcription factor (MaERF1) gene expression in banana cultivars treated with salicylic acid @ 1000 µM (which was evolved as optimum concentration of this experiment) against untreated banana was acknowledged significant results. In this current research the expression of all the genes were up regulated with the ripening process in general while it was down regulated (sometimes completely repressed) in both the cultivars treated with ethylene inhibitor salicylic acid @ 1000 μM as compared to untreated banana fruits.