Trichoderma viride is a promising biocontrol agent against major soil-borne plant pathogens like Macrophomina, Fusarium, Sclerotium, and Rhizoctonia sps, which are causing significant crop losses ranging from 25 to 45 percent. The availability of viable spore inoculum under farm-level conditions is a significant constraint for the effective management of soil-borne diseases. In addition to recycling crop residues to enhance soil health, there is an emerging need for crop cultivation. To explore the substrate for on-farm production of T. viride, an investigation was made into the effectiveness of maize shank for on-farm multiplication. The studies on the spawning technique for multiplication of T. viride using different sizes of shank such as 0.5, 1, 2, 4, 6, 8, 10 cm and powdered form of shank revealed that the powdered shank inoculated with talc formulation (20 x 106 cfu/g) of T. viride @ 10 gram in 200g shank-based spawn with 40 % moistened was found to be effective for the colonization of T. viride 28 days after inoculation.