Population dynamics of insect predators in the agroecosystem and forest ecosystem of mulberry, Morus spp., were documented in villages of Annur block, Coimbatore and at Forest College and Research Institute, Mettupalayam campus of Coimbatore district, Tamil Nadu, India, during 2020–2022. Fifteen species of predators were recorded and majority of them were coccinellids. Variations in the diversity, species richness, and evenness in the different ecosystems were studied and the result revealed that Shannon-Weiner index (3.162) and evenness index (0.98) were found to be higher in forest ecosystem compared to agroecosystem. The results also confirmed that the diversity of crop and the food availability influenced the predator population which serve as means of biological control of pest in mulberry.