Cinnamon and Tea tree oil (TTO), an essential oils and plays an important role in antibacterial activity and anti-inflammatory properties. Present study was designed for the in-vitro efficacy of essential oils, Cinnamon and Tea tree oil, against common mastitis causing organisms namely S. aureus E. coli. and Klebsiella sp. A total 360 buffaloes were screened and in- vitro efficacy of essential oils were studied by disc diffusion sensitivity test. It was observed that the antibiogram revealed that E.coli. were 100% sensitive to Cinnamon and TTO. The average zone of inhibition for cinnamon and tea tree oil was 20.50 mm±0.83, and 13.25 mm± 1.10. The zone of inhibition obtained by enrofloxacin was 22.00 mm ± 1.60. Staphylococcus aureus isolates were sensitive to Cinnamon and Tea tree oil showing zone of inhibition to the extent of 16.50 mm ± 0.56, 12.50 mm ± 0.86 respectively in comparison to 21.50 mm± 0.56 for enrofloxacin zone. The zone of inhibition against Klebsiella sp. were maximum for Enrofloxacin 20.00 mm ± 1.72 followed by Cinnamon oil 17.83mm ± 0.48 and Tea tree oil 14.17 mm ± 0.73.