This study used the detached leaf and root dip methods in order to compare the efficacy of these two methods to test pathogenicity of Fusarium oxysporum f.sp. cubense causing banana wilt. Collection and isolation of the fungus was done from infected pseudostem of the banana plant. Cultural and morphological characteristics of the isolates were observed and presence of three types of spores was noted (macroconidia, microconidia and chlamydospores). The macroconidia were slender sickle shaped while oval microconidia were observed. Pathogenicity test was performed by two different methods namely root dip inoculation method and detached leaf method. Among the two methods detached leaf method proved to be more rapid for early assessment of pathogenicity. In this method the appearance of symptoms occurs within 5-7 days after inoculation.