Globularia alypum (GA) is medicinal plant largely used traditionally in folk medcine in Algeria, locally named Tasselgha. It is effective in the treatement of various diseases. In present study, preparation of various extracts from Globularia alypum (GA) using solvent with increasing polarity were determined. Quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains the higher amount of polyphenols. However, chloroform extract (ChE) and AcE present the higher amount of flavonoids. The antibacterial effect was evaluated using the diffusion method on agar against 11 ATCC strains. AcE and ChE were more active on the most bacteria. In synergy test with Gentamicin, additive, potential, antagonist and synergic effects were found. Acute toxicity of GA was performed on male Swiss albino mice. LD50 is up to the 400 mg/kg dose of acute treatment GA extract, we did not observe significant mortality. Anti-hemolytic activity was tested of GA extracts in vitro and in vivo respectively. All extracts increased the HT50 values (Half-Hemolysis Time) in dose-dependent manner. The concentration 84.74µg/ml of ChE and AcE enhance significantly erythrocyte antiradicalar system. Administration of methanolic crude (CrE), aqeuous (AqE) and AcE with dose of 100 mg/kg/day for 21 days resulted in a significant amelioration in both blood and plasma antioxidant capacity and was confirmed in mice treated caused a highly significant (p ≤ 0.001) delay in haemolysis HT50 = 112.8 ± 2.42 min. However, EAc and EAq (100 mg/ml) caused a non-significant delay with HT50s of 69.16 ± 8.82 min and 72.35 ± 2.49 min, respectively. CrE has a moderate activity in vitro. Nevertheless, it is the best erythrocyte antiradical in vivo with a HT50 = 112.8 min. Plasma reducing power assessed by FRAP technique was used to assess the antioxidant effect of GA extracts in vivo, reducing power (RP) continues to increase after 4 minutes of reaction and even doubles at 30 minutes for all the extracts. Reaction of ascorbic acid (VIT C) did not undergo any apparent change after the first four minutes (PR4min= 0.42 ± 0.023; PR30min= 0.55 ± 0.0174). Comparison of the reducing powers of these extracts with those of the VIT C group shows that there is no significant difference Anti-inflammatory activity using phorbol myristate acetate (PMA) on mice showed that CrE reduced the weight of the ear edema. The oedema weight of the control group was 7.26 ± 0.15 mg, reduced by Diclofenac to 4.44 ± 0.13 mg. CrE of GA (100 mg/kg) also reduced the weight to 5.88 ± 0.48. Present plant had a low degree of anti-inflammatory activity (%I= 19.00 ± 3.18%) compared with that of Diclofenac used as the reference drug (%I= 38.84 ± 1.87%). In conclusion, Globularia alypum have a strong pharmacological power, which supports their medicinal traditional use.