A field experiment was conducted at Horticulture Farm, SKNCOA, Jobner during 2015-16 and 2016-17. In this study, four different varieties viz., Pusa Jwala, Pusa Sadabahar, Pusa Dipti and Amulaya and three germplasms NU-7078, NP-46A and G-S of chilli were raised and transplanted in third week of July. Roots of chilli seedlings were dipped in hundred ppm solution of salicylic acid for 30 minute before transplanting and same concentration of salicylic acid was also sprayed on all varieties before flowering and per cent disease intensity was noted 90 days after sowing. The peroxidase activity was increased (28.95%) in infected plants of Pusa Sadabahar variety followed by Pusa Depti (20.59%), NP-46A (19.35%) and Amulya (18.18%) while it was minimum in Pusa Jwala (9.09%) as compared to healthy ones. Minimum disease intensity was recorded in Pusa Sadabahar (7.93%), as it was also resulted in maximum increase in peroxidase, while higher per cent disease intensity was recorded in Pusa Jwala (43.4%), which resulted in minimum increase in peroxidase. The polyphenol content was increased maximum (23.68%) in infected plants of Pusa Sadabahar variety followed by Amulya (20.69%), NP-46A (17.14%) and it was found minimum in Pusa Jwala (13.04%) as compared to healthy ones. Phenol content was increased maximum (23.53%) in infected plants of Pusa Sadabahar variety followed by NP-46A (20.45%), G-S (14.29%), NU-7078 (12.50%) and it was recorded minimum in Pusa Jwala (12.00%) as compared to healthy ones.