Increasing levels of antimicrobial resistance in human and veterinary medicine have raised concerns around the issue of overprescribing and the indiscriminate use of antimicrobials. Antimicrobial use in food producing animals is under scrutiny due to the perceived risk from the zoonotic transfer of resistant pathogens from animals to humans. Determining significant factors that influencing veterinarian’s antimicrobial prescribing behavior can help to bridge the gap between prescribing recommendations and clinical utilization. Understanding the factors deciding prescription practices of veterinarians and its effect on their knowledge, attitude and practices towards antimicrobial resistance (AMR) is crucial in guiding efforts to curb AMR. A questionnaire study was conducted to evaluate the factors influencing veterinarian’s antimicrobial prescribing decisions in large animal practice and its impact on their knowledge, attitude and practices about AMR and stewardship among veterinarians in Kerala, India. The study findings revealed that clinical considerations influenced veterinarian’s antimicrobial prescribing decisions more than non-clinical reasons. Clinical signs/symptoms as well as the critical nature of the illness were the most often used clinical considerations in determining whether to prescribe an antimicrobial or not. Pharmacological considerations have a greater influence on antimicrobial prescribing than non-pharmacological factors. The most significant pharmacological factors were the antimicrobial's spectrum of activity, the duration of therapy and the route as well as frequency of administration. Multiple linear Regression analysis between factors influencing prescribing behavior and veterinarian’s knowledge, attitude and practices revealed that all the four factors i.e., clinical, non-clinical, pharmacological and non-pharmacological factors had a significant impact on explaining variations in veterinarian’s knowledge, attitude and practices about antimicrobial resistance and stewardship.