A field experiment was conducted for two years (rabi 2016 and rabi 2017) at SKUAST-Jammu to study the effect of differential substitution nutrients through organics on yield, nutrient uptake and profitability of knol khol (Brassica oleracea var gonylodes L.) in subtropical region of J&K. Experiment was conducted in randomized block design with sixteen treatments. The results of the study revealed that significant effect of differential substitution nutrients through organics were observed on growth parameters viz., plant height, number of leaves plant and dry matter accumulation (g/plant); yield and nutrient uptake at harvest in knol khol during both the years. Significantly highest plant height (33.14cm and 36.44cm), number of leaves/plant (15 and16.20), dry matter accumulation (28.38 g/plant and 32.65g/plant), knol khol yield (712.35q/ha and 734.79q/ha), net returns (Rs 309281 /ha and Rs 211905 /ha) were recorded with recommended dose of fertilizer during both the years where as lowest value of all these parameters were recorded with 100 % N through FYM. Significant difference between treatments was also recorded for nitrogen uptake and potassium uptake during both the years.