The continuous use of inadequate and imbalanced nutrients in the exhaustive rice-wheat systemlead to deterioration of soil fertility and soil health in Indo-Gangetic alluvial belt. In such systems, uses of concentrate organic nutrient sources as a renewable source of plant nutrients is assuming importance as organic sources integrated with inorganic fertilizersimprovescrop productivity while sustaining soil health. Therefore a field experiment was plannedto assess the cow urine effects (U2 - 12000 l ha-1 equally distributed at sowing, CRI, and spike emergence, U1 - 8000 l ha-1 equally applied at sowing and CRI and U0 - 0 l ha-1) at varying fertility (100% and 75% RDF) and zinc levels (0, 5 and 10 kg Zn ha-1) on wheat under irrigated conditionsof Varanasi in split plot design. After two years,maximum shoot dry weight m-1 row length, grain yield, straw yield, protein content, protein yield, nutrient content and uptake by grain and straw were statistically obtained with 100% RDF (F1) over 75% RDF (F2). Plants grown under 10 kg zinc ha-1resulted significantly higher values of above parameters though remained comparable to 5 kg Zn ha-1. A significant increase was obtained with respect to NPK & Zn uptake by grain and straw and protein, grain and straw yieldwith each increment of cow urine level, recording maximum at 12000 l cow urine ha-1.Within the levels of cow urine applied, 11.14% and 10.20% increase in 2016-17 and 10.31% and 9.20% increase in 2017-18 wererecorded with 12000 and 8000 l cow urine ha-1over control, respectively.