Sesame root and stem rot incited by Macrophomina phaseolina is polyphagous a soil borne and phytopathogenic fungus having a wide host range of about 500 cultivated and wild plant species worldwide. In the present study efforts were made to find out the sources of promising breeding cultivar/varieties to root and stem rot of sesame. In present investigation comprised of 314 cultivars of sesame including two local checks GT-10(resistant) and VRI-1(Susceptible) were used for rapid screened by sick pot method against Root and stem rot against M.phaseolina. Among 314 cultivars results were observed seven cultivars were recorded between 15-20 per cent and 35 cultivars recorded between 21-30 percent root and stem rot incidence.