Rice (Oryza sativa L.) plays a pivotal role in ensuring food security, particularly in Asia, where it sustains a majority of the population. Brown spot, caused by Drechslera oryzae, is a significant foliar disease affecting rice productivity, especially under rainfed and humid conditions. Effective screening and identification of resistant genotypes remain vital for developing durable resistance. In this context, a field study was conducted during Kharif 2023 at Bihar Agricultural University, Sabour, to evaluate 208 rice genotypes of the Donor Screening Nursery (DSN) for resistance to brown spot. Genotypic screening revealed substantial variation in disease reaction. None of the genotypes exhibited complete immunity (disease score ‘0’), while 17 genotypes- KNM115, KNM15361, KNM15236, 19202, 19026, 19027, 680-2, RP-Patho-1, RP-Bio-Patho-10, BPT 5204, C101LAC, C101A51, RNR 31581, RTCNP-10, RTCNP-138, NLRBL-8, and NLR 3595—were identified as resistant (disease score ‘1’). Additionally, 42 genotypes were moderately resistant, 68 moderately susceptible, 61 susceptible, and 20 highly susceptible. The identification of resistant sources through such large-scale screening forms the foundation for resistance breeding and informs integrated disease management strategies to mitigate brown spot impact in rice-growing regions of Eastern India.