Two Indian native chicken papulation, Aseel Peela (AP), Kadaknath (KN) and one exotic papulation, CARI-Red (CR) maintained at ‘Desi fowl’ unit of ICAR - Central Avian Research Institute were utilized for making full 3x3 diallel cross experiment. General immune-competence of birds (Sample size - 30 birds per genetic group) was evaluated as humoral response as well as cell mediated immunity (CMI) response. The humoral response was studied by estimating the RD-HI antibody titres by Haemagglutination inhibition test [1], while CMI response was estimated as food index [2] against the injection of mitogen (PHA-P). Full diallel analyses were done by using two models model A and model B. Higher values of foot index indicated presence of better cell mediated immunity response to PHA-P and higher values of HI titre indicate better humoral immunity against NDV. The analyses of variance for cell mediated immune response and humoral immune response revealed highly significant difference for GCA at 15 wk of ages in male, female and combined sex in case of model A and B which indicates the additive type of genetic variation. Cross KN x AP had higher and positive Heterosis percentage for cell mediated immune response against PHA-P. In case of humoral immune response against NDV cross CR x AP had highest Heterosis percentage than other crosses.