This study aimed to identify promising foxtail millet (Setaria italica L.) germplasm with superior growth vigour, yield attributes, and disease resistance for cultivation in the mid-hills of Uttarakhand, and to select elite lines for breeding purposes. A total of 28 germplasm lines, including a local check (PRK-1), were evaluated during Kharif 2025 under field conditions using a Randomized Block Design with three replications at the B-Block Research Field, Department of Plant Pathology, College of Hill Agriculture, Ranichauri, Tehri Garhwal, under V.C.S.G. Uttarakhand University of Horticulture and Forestry, Bharsar, Uttarakhand. Observations were recorded on important growth and yield traits, and the germplasm was screened against major endemic diseases using standard rating scales. Genetic parameters were estimated to assess variability among the lines. Analysis of genotypic, phenotypic, and environmental variances, along with coefficients of variation, revealed substantial variability among the genotypes, indicating considerable scope for selection. Most traits exhibited high heritability (>75%) coupled with moderate to high genetic advance, except seed yield, 1000-seed weight, and number of tillers. A close association between genotypic and phenotypic coefficients of variation indicated minimal environmental influence on trait expression. Significant morphological and physiological variation was observed among the germplasm, with GP-12, GP-23, and GP-26 showing superior growth vigour and yield performance. Lines GP-6, GP-7, GP-12, GP-23, and PRK-1 exhibited high levels of disease resistance, particularly against leaf blast, leaf blight, sheath blight (<5.67%), and grain smut (<3.3%). These germplasm lines may serve as valuable resources for developing climate-resilient, high-yielding cultivars for the mid-hill regions of Uttarakhand.