The present investigation was undertaken with a view to generate genetic information on gene effects for seed cotton yield per plant in cotton (Gossypium hirsutum L.) using 21 generations. Special scaling tests such as (B1-L1), (B2-L2) and (F2-L3) were significant either in cross 1 or cross 2 for all the four traits besides significance of other tests showing presence of epistasis. The 𝜒2(2) value at 15 degrees of freedom were significant in all the traits in both crosses supported the presence of higher order epistasis and /or linked digenic interaction. The 𝜒2(3) value at 11 degrees of freedom was significant for all the four traits in both the crosses indicating the presence of higher order epistasis. The 𝜒2(4) value at 9 degrees of freedom was significant for all the four traits in both the crosses indicating the presence of higher order linkage.