Peppermint (Mentha piperita L.) is mainly cultivated during the spring season for its medicinal and quality uses. The present investigation was planned to evaluate the row spacing and nitrogen application rate to ensure potential herbage yield and superior quality essential oil. The experiment was laid out in split plot design at two locations (Ludhiana and Bathinda) keeping combinations of Two varieties (Local and Kukrail) and three row spacing (30 cm, 45 cm and 60 cm) in main plots and four nitrogen levels (0, 50, 75 and 100 kg/ha N) in sub plots. The Kukrail variety performed significantly better than Local variety in terms of plant height, stool count, leaf area index resulting in 7.47 % and 7.69 % higher herbage yield at Ludhiana and Bathinda, respectively. Planting of peppermint at 45 cm row spacing produced significantly higher growth parameters like plant height, dry matter and fresh herb yield than 30 cm while the differences between 45 and 60 cm were non-significant. Also, the application of 75 kg/ha N produced significantly higher herb and oil yield than 0 kg/ha N and 50 kg/ha N while the response beyond 75 kg/ha N was non-significant at both the locations.