Given the versatility and economic significance of tuberose, especially for small and marginal farmers, it is essential to understand its response to abiotic stresses. Salt stress is a major factor that adversely impacts plant growth, yield and quality. Despite extensive research on other crops, the effects of salinity stress on tuberose remain largely underexplored. Present study aims to address this gap by evaluating the differential responses of tuberose genotypes to salinity stress. 18 tuberose genotypes were subjected to salinity stress at EC levels of 2, 4, 6 and 8 dS/m, along with control. The results demonstrated a significant reduction in all measured parameters with increasing salinity levels. Tuberose genotypes showed markedly varied responses to different levels of salinity stress. ‘Pearl Double’ exhibited the longest spike length even under highest salinity level (69.38 cm at 8 dS/m). Additionally, ‘Pearl Double’ showed minimum percentage reduction in leaf width, with reductions of 1.53%, 4.08% and 17.35% at salinity levels of 4 dS/m, 6 dS/m and 8 dS/m, respectively, indicating its superior tolerance to salinity. ‘Prajwal’ and ‘Bidhan Snigdha’ also displayed significant tolerance to higher salinity stress. Conversely, ‘Arka Shringar’ and ‘Mexican Single’ showed highest percentage reduction when exposed to salinity stress. Among studied genotypes, ‘Pearl Double’, ‘Bidhan Snigdha’ and ‘Prajwal’ emerged as promising genotypes, displaying notable tolerance and minimal percentage reduction under salt stress conditions. Conversely, ‘Arka Shringar’ and ‘Mexican Single’ were found to be susceptible genotypes to salinity stress.