Millet constitutes one of the most reliable dry-season crops in terms of global agricultural production. Pearl millet is the main millet crop grown in India. The present study was undertaken to investigate the physico-chemical composition of five different varieties of pearl millet. The evaluation of five different varieties viz; Pusa composite (383), Pusa composite (701), Dhanshakti, MBC-2 and Local variety-ACRA for physical parameters (seed weight, seed density, swelling capacity and swelling index), chemical parameters (moisture, crude protein and ash content) and antinutritional parameters (phytic acid and polyphenols) was carried out. The highest seed weight (11.87 g), seed density (1.24 g/ml), swelling capacity (0.65 5) and swelling index (0.55 %) was exhibited by Dhanshakti variety, whereas the lowest was observed in Local variety-ACRA. Maximum moisture content was observed in Local variety-ACRA (11.23 %), crude protein and ash content (11.13 and 1.87 %), respectively recorded in Dhanshakti. In contrast, the highest values for phytic acid (765.96 mg/100 g) and polyphenols (601.73 mg/100 g) were found in Local variety-ACRA.