The leaf quality characters were studied in thirteen mulberry accessions viz., ME-27, Srinagar local, C-776, Karanahalli, MI-79, Morus indica, SB-21, V-1, S-30, ME-144, MI-139, MI-506 and TB-21. V-1 was taken as control. The moisture content (76.05%), moisture retention capacity (MRC) after 3 hours (75.88%), 6 h (72.85%), 9 h (69.75%), and 24 h (61.26%) were significantly maximum in TB-21. MRC after 12 h (66.86%) was higher in V-1. The moisture content and MRC were minimum in MI-506 and Srinagar local. The biochemical analysis of leaves reveled that total nitrogen, crude protein, protein, total amino acids, starch, sugar, total carbohydrates, total chlorophyll content were found to be high in TB-21, ME-27 and MI-79, whereas they were low in Srinagar local and C-776. Crude fibre was higher in ME-17 and ME-144. Total ash was higher in TB-21 and MI-79. Hence, TB-21, ME-27 and MI-79 can be further evaluated in preliminary yield trial for growth, yield and silkworm rearing performance.