Lentinula edodes (Berk.) Peglar, the shiitake mushroom, is worldwide one of the most widely cultivated mushrooms. The cultivation of edible mushrooms is a biotechnological process that uses various residues to produce food of high nutritional value. Two strains of Lentinula edodes (DMR-356 and DMR-35) were cultivated on basal substrate wheat straw and poplar sawdust alone and in combination with supplements (Wheat bran, Rice bran and Maize Meal). Complete spawn run, bump formation, basideocarp formation days, total yield and biological efficiency were recorded. Minimum time taken for complete spawn run, bump formation and basideocarp formation was observed on wheat straw + wheat bran (20%) + CaCo3 (2%). Wheat straw + wheat bran (20%) + CaCo3 (2%) produced maximum total yield and biological efficiency in DMR-356 (567.0g/900g of dry substrate) with biological efficiency (63.0%) and DMR-35 (497.6g /900g of dry substrate) with biological efficiency (55.2%). Poplar sawdust substrates alone proved to be least effective for the cultivation of Lentinula edodes strains, with maximum days taken for complete spawn run, Bump formation and basideocarp formation day, while minimum total yield with DMR-356 (114.0g/ 900g of dry substrate) and corresponding lowest biological efficiency (12.6%) and with DMR-35 strain minimum total yield (57.0g/ 900g of dry substrate) and biological efficiency (6.0%) was recorded.