Mulberry is a multipurpose fruit and it’s gaining importance as its in house of health promoting bio compounds viz., anthocyanins, phloridzin, quercetin, chlorogenic acid, resveratrol and rutin. The pharmaceutical and nutraceutical properties of mulberry fruits led to the investigations on the antioxidant activity of mulberry fruits to find promising natural anti oxidants. Though, the mulberry fruit is rich in variety of functional components, it is not sufficiently investigated. However, limited studies are reported on the antioxidant activity. Hence, the present investigation was made on antioxidant activity of mulberry fruits viz., DPPH radical scavenging activity, ABTS Radical scavenging activity and FRAP Radical scavenging activity. Different concentrations viz., 10, 20, 40, 60, 80 and 100µg/ml of fruits extracts of MR2 and G4 variety were used for the study. The results indicated that mulberry fruit extract possess higher antioxidant activity than standard which could be exploited for commercial natural antioxidants in food, pharmaceutical and cosmetic industries.