In order to the treat increasing diseases in a milieu of global emergence of antimicrobial resistance, sustainable eco-friendly alternatives are urgently needed. Thus, in-vitro evaluation of the therapeutic feasibility and employability of environmental- friendly compound, a combination of dolomite and ethanolic extract of turmeric in the ratio 9:1, against aquatic environmental isolate (antibiotic resistant and sensitive) of Aeromonas hydrophila was performed. The bacteria were isolated and characterized from fish farms in Ernakulam district, Kerala. The antimicrobial activity of the compound was assessed by the resazurin based broth microdilution to determine minimum inhibitory concentration (MIC). Time-kill kinetics assay performed at varied concentrations of 1xMIC,2xMIC and 4xMIC concentrations. Also, the crystal-violet based biofilm inhibition assay was performed. Exposure study was performed at sub-lethal concentration of 0.5xMIC. MIC of the combination was found to be at 64 µg/ml and 4096 µg/ml against the sensitive and resistant A.hydrophila respectively. Time-kill kinetics assay showed the bacteriostatic effect of the compound. Biofilm inhibition assay confirmed the ability of compound to reduce the biofilm formation propensity of the bacterial community. The exposure study performed at sub-lethal concentration of 0.5xMIC showed no changes in the resistance pattern. The results of the study validate an eco-friendly alternative for antibiotic use in aquaculture.