Journal ID : AMA-13-09-2022-11673
[This article belongs to Volume - 53, Issue - 09]
Total View : 458

Title : Evaluation and Molecular Characterization of Native Fluorescent Pseudomonads against Fungal and Bacterial Wilt of Brinjal

Abstract :

Brinjal (Solanum melongena L) is one of the most common vegetable crops grown in tropical & subtropical regions of the world. Brinjal cultivation is affected by various pest and diseases. The soil borne diseases like fungal and bacterial wilt are the most destructive diseases of brinjal. These are caused by Fusarium oxysporum f.sp. melongenaeSchlecht (Fungal wilt)Ralstonia solanacearum (Smith) Yabuuchi et al. (Bacterial wilt). Under the present investigation two isolates of FluorescentPseudomonads (FLPs) viz., FLP-Brinjal 2020-1 and FLP-Brinjal 2020-2, were evaluated against two major pathogens of brinjal viz., Fusarium oxysporum f.sp. melongenae (FOM) andRalstonia solanacearum(RAL)under in vivo and in vitro conditions using dual culture and inverted plate assay. FLP-Brinjal 2020-2 gave maximum inhibition over the control followed by FLP-Brinjal 2020-1 against both the pathogens. Experiments conducted in pluck trays indicated that FLP-Brinjal 2020-2 was superior with maximum germination percentage (63.75%), vigour index (650), disease control percentage (58.66%), bio-control efficiency, plant growth (10.20 cm) and minimum disease incidence (20.62%) against bacterial wilt pathogen. Similarly, under brinjal fungal wilt experiment maximum bio-control efficiency, plant growth (11.20 cm) and minimum disease incidence (%) (24.81%) among the Fusarium challenged treatment was found with FLP-Brinjal 2020-2. The identity and diversity among the FLP isolates were examined by sequence analysis of 16s rRNA gene. The gene was amplified by PCR using primers pair, 27 F/1492 R. Amplified DNA products were sequenced, and analysed by BLAST programme at NCBI database for species identification. Among the 10 FLP isolates assessed, 9 were found to have maximum similarity with Pseudomonas aeruginosa includingFLP 2020-2 and FLP 2020-1 was found to be Pseudomonas fluorescence which is in confirmation with the morphological observations.

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