Mentha piperita L. (Peppermint) is a perennial glabrous and strongly scented herb belonging to family Lamiaceae. The plant is aromatic, stimulant and used for allaying nausea, headache and vomiting. Its oil is one of the most popular widely used essential oils in food products, cosmetics, pharmaceuticals, dental preparations, mouthwashes, soaps and alcoholic liquors. The study was carried out in Community Science College and Research Institute, Madurai. The phytochemicals such as alkaloids, tannins, phenols, flavonoids and steroids were analyzed in the pepper mint leaf extraction. The different solvents such as ethanol, methanol, chloroform, hexane and distilled water were used for extraction of leaves. Among the solvents hexane and chloroform extracts had more amounts of alkaloids was present. Followed by other solvents such methanol, ethanol and distilled water had some amounts of alkaloids. The total phenol content of fresh sample (693.3 mg) is higher than the powder sample (666.6 mg) and the tannin content of fresh sample is higher (866 mg) than the powder sample (543 mg). The antioxidant activity of fresh sample (8.1% in ml leaf concentration) and powder sample (22%) were analyzed. Radical scavenging activity antioxidant activity of powder sample is higher than the fresh sample.