The objective of the study was to isolate and identify enteric pathogens, specifically Salmonella and Escherichia coli, in captive Asian elephants (Elephas maximus) in Tamil Nadu state. Water and dung samples were collected from six different locations in Tamil Nadu. Standard culture methods and PCR techniques were used to isolate and identify Salmonella and E. coli in both water and dung samples. Out of the six enteric samples, 100% (n=6) tested positive for Salmonella and E. coli. Among the dung samples (n=6) and water samples (n=6) examined for enteric cases, 83.33% (n=5) of water samples and 83.33% (n=5) of dung samples tested positive for E. coli, while 16.67% (n=1) of water samples and 16.67% (n=1) of dung samples tested positive for Salmonella. Additionally, 16.67% (n=1) of water samples and 16.67% (n=1) of dung samples tested positive for a combination of E. coli and Salmonella. The presence of red-colored colonies on Brilliant Green Agar and colorless colonies with black spots at the center indicated the growth of Salmonella. On MacConkey Agar, lactose-fermenting pink-colored colonies with a foul smell and colonies with blackish centers and a metallic sheen on Eosin-Methylene blue agar indicated the growth of E. coli. Molecular identification of Salmonella and E. coli was performed using Polymerase Chain Reaction (PCR), resulting in amplicon sizes of 260 and 476 base pairs (bp) for Salmonella and E. coli, respectively. These findings highlight the importance of providing high-quality water to captive elephants to minimize the risk of contamination with microorganisms such as E. coli and Salmonella.