To test the effects of genotypic diversity on natural enemy population of soybean insect-pests an experiment was laid in kharif 2018 and 2019 at the Entomology Research Farm of ICAR-Indian Institute of Soybean Research, Indore. Totally 20 treatments were chosen for this experiment and out of the twenty treatments four treatments namely, T1, T6, T11 and T16 were four varietal mixture treatments. The results of the effects of genotypic diversity on natural enemy population of soybean insect-pests were revealed that highest population of coccinellids (2.32 coccinellids/m2) and spiders (11.74 spider/ m2) were found in treatment T16 (mixture of NRC-37, JS-9752, RSC-1046 and RKS-113) while rove beetle (2.54 rove beetle / m2) was found in treatment T1 (Mixture of JS-9560, JS-20-34, MAUS-47 and MACS-1460). Canthconidia (6.67 Canthconidia/m2) and Hover fly (11.74 hover fly/m2) were found highest in treatment T6 (JS-9305, JS-2029, RVS-2001-4 and Dsb-28-3) while predatory thrips (2.44 predatory thrips) was found in T11 (mixture of NRC-86, JS-335, JS-2098 and RKS-45). For insect pathogens the highest infected larva of defoliators viz., semoloopers and Spodoptera litura etc. by entomopathogenic fungi, Beaveria basiana (4.61 infected larva/m2) entomopathogenic fungi was found in T11 while Nomuriya rileyi (10.67 infected larva/m2) infected defoliators larva was found in T16. In case of parasitoids i.e., Encarsia transvenna (22.87 parasitized pupae/leave) (whitefly parasitoids) and braconids (3.69 parasitized pupae/leave) (lepidopterous parasitoid) were found highest in T16 (mixture of NRC-37, JS-9752, RSC-1046 and RKS-113). So, it is evident from above results that genotypic diverse treatments (varietal mixtures) have more number of all kinds of natural enemies than their respective sole varietal treatments.