The effect of forty six (46) different soybean genotypes on the biology and food consumption and nutritional indices of Spodoptera litura (Fabricius) (Noctuidae:Lepidoptera) have been studied. Differences in various biological parameters viz., incubation period, larval duration, pre pupal period, pupal period, longevity of adults) of S. litura were observed on different genotypes. The shortest larval, pre pupal and pupal period was found in larvae reared on CAT 2503 (13.19 days) and F3P18 (1.23 and 7.07 days), respectively. The shortest pre-oviposition and oviposition period was found in larvae reared on CAT 1847 (2.02 and 3.05 days), post-oviposition period in CAT 488 (2.75 days), respectively. The total life period of male was found larvae reared on in F4P21 (28.15 days) and female was in CAT 488 (31.20 days), respectively. Longevity of both male and female was observed in F3P18 (6.64 and 9.72 days, respectively). The lowest per cent adult emergence, sex ratio, fecundity and incubation period was observed in F4P21 (68.40), F3P18 (1:1.19 and 216.8 eggs) and CAT 349 (2.56 days), respectively. The lowest weight of 3rd instar, 4th instar, 5th instar larva, pre pupa and pupa was found larva reared on F4P21 (0.83 gms), NRC-138 (1.04 gms), PS1569 (1.08 gms), RVSM 2012-4 (0.52 gms) and CAT2503 (0.66 gms), respectively. The lowest Approximate Digestibility (AD), Efficiency of Conversion Digested Food (ECD), Efficiency of Conversion Digested Food (ECI) and Weight gain (Wg) by larva was found lowest in larva reared on CAT488 (56.60%), F4P21 (18.30%), CAT349 (24.25%) and F3P18(0.72 gm), respectively.