Exploration of nanoparticles (NPs) in the field of agriculture opened a great and remarkable attention because of its unique propensity and high surface reactivity. The efficacy of different metallic silver and copper nanoparticles were used to determine seed germination, growth and biochemical properties citron (Citrus medica L.). Citron seeds were treated with metallic AgNPs (<90nm) and CuNPs (50nm) suspension using stabilizing agents poly ethylene glycol (PEG) @ 6000 ppm to prevent particle aggregation and electrostatic repulsion and incubated for eight hours at 225 rpm in an orbital shaker. The seeds treated with AgNPs enhanced the germination percentage (98.33–100.00%), mean germination time (29.25–33.25 days), germination rate of seeds per day (1.89–2.10) and germination index (4.77–5.33) as compared to CuNPs and non-treated seeds. The seedlings kept under protected condition performed better than open condition. AgNPs @ 20 ppm showed the maximum plant height (13.94 cm and 14.20 cm under open and protected condition respectively), maximum root length (32.51cm in open and 33.67 cm in protected condition), leaf emergence rate (10.33 under open and 10.87 under protected) and maximum fresh weight (5.00 g) and dry weight (1.57 g) of seedlings at120 days after germination (DAG). AgNPs @ 5 ppm improved total chlorophyll content (0.80 mg g-1) in leaves. The highest total phenolics (8.59 mg GAE 100g-1) content in leaves was recorded in CuNPs @ 40 ppm.