Effect of plant growth regulators on shelf life of guava (Psidium guajava L.) under high density plantation was carried out at Research Farm, SKUAST- Jammu, Chatha during 2021-2022. There were 9 treatments comprising of foliar application of NAA @ 100 and 150 ppm, GA3 @ 75 and 150 ppm, applied individually, NAA 100 ppm in combination with GA3 75 ppm, NAA 100 ppm in combination with GA3 150 ppm, NAA 150 ppm in combination with GA3 75 ppm, NAA 150 ppm in combination with GA3 150 ppm and control (spray). During investigation it was observed that all physical and biochemical parameters of guava showed a decreasing trend with advancement in storage intervals. Application of NAA 150 ppm in combination with GA3 150 ppm recorded minimum mean physiological loss in weight of guava fruit (4.69 %), Highest mean rotting percentage of guava fruits (8.89 %) , Maximum mean fruit length (6.85 cm), Maximum mean fruit breadth (6.85 cm),Maximum mean fruit weight (140.28 g), Maximum mean fruit volume (134.29 cc), Maximum mean fruit firmness (6.75 kg/cm2),Highest mean TSS of guava fruits (10.66° Brix), Maximum mean ascorbic acid (173.39 mg/100g fresh wt.), Lowest mean titratable acidity (0.31 %), highest mean organoleptic rating of 6.87. Overall, it was concluded that NAA 150 ppm plus GA3 150 ppm resulted in longer shelf life when stored under optimum conditions.