An experiment was conducted on medium black clayey soil at organic plot, Krishi Vigyan Kendra, JAU, Amreli (Gujarat) during rabi seasons of 2018-19 and 2019-20 to evaluate cow-based bio-enhancers and botanicals for organic cultivation of onion (var. GJRO-11). Ten treatments comprising Panchagavya as foliar spray @ 3% at 30, 45 and 60 DAT + FYM @ 5 t ha-1, Jivamrut as drenching @ 5% at 15, 30 and 45 DAT + FYM @ 5 t ha-1, Cow urine as foliar spray @ 3% at 15, 30 and 45 DAT + FYM @ 5 t ha-1, Seaweed extract as foliar spray @ 3.5% at 30, 45 and 60 DAT + FYM @ 5 t ha-1, Banana sap as foliar spray @ 1% at 30, 45 and 60 DAT + FYM @ 5 t ha-1, Vermiwash as foliar spray @ 2% at 45 and 60 DAT + FYM @ 5 t ha-1, Enriched vermi compost @ 2 t ha-1 + FYM @ 5 t ha-1, Vermi compost @ 2 t ha-1 + FYM @ 5 t ha-1, FYM @ 20 t ha-1 and Control (Absolute) in randomized block design with three replications. The experiment results on the basis of two years pooled mean revealed that application of FYM 20 t ha-1 followed by Panchagavya as foliar spray @ 3 % at 30, 45 and 60 DAT + FYM @ 5 t ha-1, Jivamrut as drenching @ 5% at 15, 30 and 45 DAT + FYM @ 5 t ha-1, Enriched vermi compost @ 2 t ha-1 + FYM @ 5 t ha-1, vermi compost @ 2 t ha-1 + FYM @ 5 t ha-1 were found superior in nutrient content.