Field experiment were carried out to investigate the effect of organic manures and iron on alkalinity tolerance of wheat under alkali water irrigation in rabi 2019-20 and 2020-21 at SKNAU Jobner Jaipur. As per results irrigation with 10 mmol L-1 RSC water significantly reduced the ionic regulation index for Potassium (IRI-K) (1.145), increased ionic regulation index for sodium (IRI-Na) (4.159), degree of compartmentation at flowering stage (11.20) and higher Na/Ca, Na/K and Na+K/Ca ratios in grain and straw during both the study years and pooled mean in wheat crop. Whereas, significantly maximum Ca/Mg ratio in wheat grain (3.289) and straw (1.798) was observed at 2 mmol L-1 RSC water in irrigation during both the study year and pooled analysis. At flowering stage ionic regulation index for Potassium (IRI-K) increased significantly with application of 5 t ha-1 vermicompost and 15 t ha-1 FYM and 100 kg ha-1 FeSO4.7H2O while, ionic regulation index for sodium (IRI-Na) and degree of compartmentation decrease considerably in both the years and pooled mean and lower Na/Ca, Na/K and Na+K/Ca ratios in grain and straw during both the years and pooled in wheat crop. Whereas, significantly higher Ca/Mg ratio in grain was reported with of 5 t vermicompost ha-1 and 15 t FYM ha-1 and 100 kg FeSO4.7H2O ha-1 application during both the study year and pooled analysis. However, the effect of FYM and vermicompost application was found to be at par with each other.