A pot experiment was conducted to evaluate the effect of organic amendments on reduction and phyto-availability of chromium in mustard crop (Brassica juncea L.) during 2017-18 at Banaras Hindu University, Varanasi. Different organic amendments sources viz. FYM, vermicompost, poultry manure and sewage sludge were tested at four level of chromium (0, 50, 75 and 100 ppm) total 20 treatment combinations in complete randomized design and replicated thrice. The application of 100 ppm Cr + PM @ 5 ton ha-1 recorded significantly minimum of chromium content in root, shoot and seed of mustard (19.66, 11.63 and 2.127 mg kg-1, respectively), chromium content in post harvest soil (0.833 mg pot-1) and transfer factor (TF) of root, shoot and seed of mustard (21.16, 7.02 and 0.437, respectively) then other treatment combinations. The application of 100 ppm Cr + PM @ 5 ton ha-1 recorded significantly higher of Cr accumulation in root, shoot and seed of mustard (36.91, 61.16 and 0.550 mg pot-1, respectively) and translocation ratio of shoot and seed of mustard (0.634 and 0.113, respectively) then other chromium level and organic amendment combinations.