To study the effect of melatonin during different seasons on some hormonal and physiological responses in goats, twenty four (24) apparently healthy, non-pregnant, non-lactating goats, having approximately similar body weight and age group reared under uniform managemental husbandry practices was selected from Livestock Farm Complex, College of Veterinary Science and Animal Husbandary, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya. The experiments were carried out in spring, summer, autumn and winter seasons in the year 2023. 5 ml/animal blood sample with and without anticoagulant in vials was collected at 0 day, 7 day, 15 day, and 30 day. Anticoagulant added blood was used for Plasma collection. Plasma cortisol, plasma estrogen and plasma progesterone was found significantly (P<0.05) higher in T1,T2 and T3 as compared to control in spring season and similar result was found in summer, autumn and winter season.