To study the effect of melatonin during different seasons on some haemato-biochemical responses in goats, twenty four (24) apparently healthy, non-pregnant, non-lactating goats, having approximately similar body weight and age group reared under uniform managemental husbandry practices was selected from Livestock Farm Complex, College of Veterinary Science and Animal Husbandary, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya. The experiments were carried out in spring, summer, autumn and winter seasons in the year 2023. Blood sample with and without anticoagulant in vials was collected at 0 day, 7 day, 15 day, and 30 day. Blood sample was analyzed for haematological parameters (Hb, PCV, TEC and TLC) and different biochemical parameters like total protein and total immunoglobulin was also analyzed. Plasma total protein and total immunoglobulin was found significantly (P<0.05) higher in T1,T2 and T3 as compared to control in spring season and similar result was found in summer, autumn and winter season. Haemoglobin concentration, Packed Cell Volume (PCV), Total Erythrocyte Count (TEC) and Total Leucocyte Count (TLC) were significantly (P<0.05) higher in spring as compared to control and similar result was found in summer, autumn and winter seasons.