The present investigation was undertaken to study the “Effect of IBA and rooting media on air layering of Kagzi lime (Citrus aurantifolia Swingle) under rainfed conditions” at the Rainfed Research Sub-Station for Sub-Tropical Fruits (RRSS), Raya, SKUAST- Jammu during the rainy season of 2020-2021. Juvenile branches with 1.00 to 2.00 cm in diameter were girdled and treated with rooting hormoneIndole-3- butyric acid (IBA) @(500 ppm, 1000 ppm and 1500 ppm), four levels of rooting media in different ratio viz., soil + sphagnum moss (2:1); soil + cocopeat + vermicompost (2:1:1) and soil + F.Y.M + sand (2:1:1) along with the control (soil)and their combinations were investigated. Callus was formed at the girdled portions on all air layers with or without the application of hormone. Results revealed that interaction effect of growth regulator and rooting media, IBA@1500 ppm + soil and sphagnum moss (2:1) proved significant in maximizing success percentage (%) of layered plants (95.99%), root parameters i.e. number of primary roots (30.67), number of secondary roots (40.00), maximum length of primary roots (10.70 cm), maximum length of secondary roots (10.27 cm) and survival percentage of air layered plants (98.68%) after planting, whereas, the minimum was reported in control.