An experiment of five w weeks duration was conducted at Livestock Farm Complex, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya to access the impact of histidine supplementation on growth performance, hemato- biochemical profile and economics of broiler production. Total 200 day old chicks were randomly divided into four dietary treatment and each group was further redistributed into five replicates containing 10 chicks in each i.e. control group (T1) with basal diet regimen, T2 group (Basal diet+ Histidine @0.05%), T3 group (Basal diet + Histidine @ 0.10%), T4 group (Basal diet+ Histidine @0.2%). Body weight, body weight gain, average daily gain, growth rate, feed intake, gain feed ratio, performance index, hemato-biochemical profile and economic efficiency were assessed. In the third, fourth and fifth weeks T3 group broilers body weight were significantly (P<0.05) higher than T1, T2 and T4 group. The average total feed intake as well as daily feed intake during the entire experimental period were significantly (P<0.05) lower in T3 group, with respect of control group. The T3 group birds had significantly (P<0.05) higher gain to feed ratio and performance index, as compared to control group. The serum glucose, total protein, albumin and globulin values were significantly (P<0.05) higher in histidine supplemented birds in all the dose regimen, as compared with control group. The cost of production of T1, T2 and T3 groups birds were comparable (P>0.05) but significantly (P<0.05) higher than T4 group. The birds supplemented with 0.10% histidine had significantly (P<0.05) higher feed price ratio, profitability and economic efficiency index, as compared with control group. The observation of break even output was lowest (P<0.05) in T3 and highest (P<0.05) in T4 group. So, it might be concluded that, the dietary supplementation of histidine @ 0.10 % has ability to improve the performance of broiler chickens.