Assam lemon is one of the important Citrus species. It is indigenous to Assam. Main storage problems of lemon fruits are browning, shrinking of peel, loss of green colour, loss of firmness, and reduced juice content. Providing non-hazardous edible coating would permit the possibility to reach distant markets in fresh form. Use of CMC (Carboxy Methyl Cellulose) as exterior coating along with Sodium Benzoate and Bees Wax coating can increase shelf life of Assam Lemon. The present investigation was carried out in Laboratory, Department of Horticulture, Assam Agricultural University, Jorhat during the year 2022-2023. Treatments includes combination of Carboxy Methyl Cellulose, propylene glycol, sodium benzoate. Fruits were stored in corrugated fibre boxes. Physical and biochemical evaluation was done at every 5 days interval. Different observations were recorded where highest juice content was recorded in T₅ (39.59%), highest TSS recorded in T₅(6.270Brix), highest titratable acidity recorded in T₅(3.42%) and lowest physiological loss in weight was observed in T₃ on 25th day of storage. The results of the experiment revealed that application of treatments had significant effect on various biochemical parameter of lemon fruit during storage. Treatment T₅ (3% CMC + 2% Propylene Glycol + Sodium Benzoate 500 ppm) showed better results followed by T₃ (5% CMC + 2% Propylene Glycol) during storage. T₅ (3% CMC + 2% Propylene Glycol + Sodium Benzoate 500 ppm) was superior as an edible coating and retained better quality with extended storage life as compared to all other treatments.