Nicotine, a potent toxicant in cigarette smoke, adversely affects various stages of female reproductive function by acting as a pro-oxidant, increasing the generation of reactive oxygen species (ROS). Plant-derived antioxidants, known for their exceptional free radical-scavenging abilities, were the focus of this study. The primary objective was to explore the antioxidant effects of green-synthesized silver nanoparticles, termed Green nanocolloid-CcAgNps, on in vitro cultured buffalo oocytes, with the goal of mitigating nicotine’s impact. The assessment relied on the maturation rate of oocytes and the quantitative RT-PCR analysis of genes associated with antioxidant enzymes (SOD1 and GPX4), anti-apoptotic factors (BCL2) and fertility-related genes (GDF-9). Results showed a significantly higher maturation rate of oocytes cultured in a medium containing both nicotine and green nanocolloid than those exposed to nicotine alone. Moreover, GPX4 expression markedly increased in the nicotine with green nanocolloid group compared to the control group. Nicotine in the maturation medium led to a substantial upregulation of BCL2 and GDF-9 gene expression, while the addition of green nanocolloid alongside nicotine resulted in a significant downregulation of RNA expression. This indicates that the presence of green nanocolloid reduces the damage caused by nicotine and the study suggests that oocyte maturation rates improve without reliance on an enzymatic antioxidant system. This improvement is likely due to the potent free-radical scavenging capacity of silver nanoparticles capped with Cymbopogon citratus.